Cartwright N Demaine A Jahromi M Sanders H Kaminski ER.
A study of cytokine protein secretion, frequencies of cytokine expressing cells and IFN-G gene polymorphisms in
normal individuals, Transplantation 68(10) :1546-52, 1999.
Abstract
BACKGROUND: Cytokines are major regulators of immune responses, and there is evidence that they play a role in allograft rejection.
Before embarking on a detailed study of pretransplant cytokine profiles in renal allograft recipients, we wished to investigate variations in
cytokine protein secretion, numbers of cytokine expressing T cells, and cytokine gene polymorphisms in normal volunteers. METHODS:
Twenty normal healthy volunteers were studied. Cytokine protein secretion [interleukin- (IL) 2, IL-4, IL-10, and interferon- (IFN) y] and
numbers of cytokine expressing CD3+ T cells (IL-2, IL-4, IL-10, and IFN-gamma) were quantified by means of enzyme-linked
immunosorbent assay and two-color flow cytometry respectively. IFN-gamma gene polymorphisms were determined by polymerase chain
reaction and autoradio graphy. RESULTS: Large interindividual variations in both the quantity of IL-2, IL-4, IL-10, and IFN-gamma cytokine protein secreted and numbers of IL-2 and IFN-gamma expressing T cells were demonstrated.
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