Jeohn GH, Kong LY, Wilson B, Hudson P, Hong JS.
Synergistic neurotoxic effects of combined treatments with cytokines in murine primary mixed neuron/glia
cultures, J Neuroimmunol 85(1) :1-10, 1998.
Abstract
Activation of brain glial cells with the bacterial endotoxin lipopolysaccharide (LPS), the HIV-1 coat protein gp120, or beta-amyloid-derived
peptides, stimulates the expression of several cytokines, including tumor necrosis factor-alpha (TNFalpha), interleukin-1 (IL-1) and IL-6.
and nitric oxide (NO) which have been proposed as causes of neurodegeneration in the brain. In the present study, the neurotoxic effects of
several cytokines, alone or in various combinations, and the correlations of the release of lactate dehydrogenase, the loss of neurons, and
the secretion of NO in brain neuronal cell injury were investigated in murine primary mixed neuronal/glial cell cultures.
Important points:
-Model: neuron/glia culture, mouse
-measured by LDH, only one specific combination of the cytokines IL1-a, TNF-a, IL-6, and IFN-gamma was toxic: IL-1 (1 ng/ml)+ TNFalpha (10 ng/ml)/interferon-gamma (IFNgamma) (200 u/ml)
-timing: > 48h
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